By Henrik Nielsen (auth.), Henrik Nielsen (eds.)
Recent perception into the transcripts generated from the mammalian genome (i.e. the transcriptome) has printed that transcription is a much more complicated phenomenon than formerly idea. In RNA: tools and Protocols, specialist researchers give you the strategies and techniques used to explain the constitution of messenger RNAs and non-coding RNAs which are transcribed by way of RNA polymerase II because the instant gene items in mammalian cells. occupied with the constitution of the RNA items of “gene X” and the mapping of proteins linked to those RNAs, the quantity provides acceptable details for non-specialists in RNA biology. Written within the hugely winning equipment in Molecular Biology™ sequence layout, many chapters include introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and key tips about troubleshooting and heading off recognized pitfalls. accomplished and useful, RNA: tools and Protocols perspectives the transcriptional panorama with an appreciation for the position that proteins play within the processing and interpretation of genetic details in an try and additional our the most important wisdom of the numerous items and complex regulatory networks that outcome from it.
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