Download E-books Cytokine Protocols (Methods in Molecular Biology Vol 249) PDF

By Marc De Ley

A suite of biochemical, mobile, and molecular strategies for unraveling and quantifying the occasions happening among the preliminary touch of a cytokine on the membrane receptor and the eventual activation of gene transcription. The options used comprise the new release of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights comprise RT-PCR of small quantities of mRNA, in situ hybridization, biosensor research, dimension of organic actions and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization.

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